This role will be responsible for designing, performing, analyzing, and documenting experiments to support mammalian synthetic biology activities within Resilience R&D. They will work closely to assist scientists & engineers to develop and test new experimental hypotheses and technical workflows. The ideal candidate will have a proven track record of successfully developing molecular biology tools with experimental biologists and bioinformaticians. They will serve as a technical lead that coordinates day-to-day research efforts of several research associates.
Independently design and execute genome engineering and cell design experiments.
Working with scientists and engineers, perform cell-based and in-vitro experiments on new gene and protein designs.
Track new techniques and technologies in the field of synthetic biology (i.e. inducible promotors, kill-switches, and novel functionality).
Prepare presentations and present scientific topics and experimental methods you are working on.
Develop, implement, and improve protocols and procedures based on experimental data and optimizations.
Ensure documentation and data management practices are followed, reviewing and revising documentation frequently.
Support the research team's knowledge curation with independent literature review and study.
Assist with organizing and streamlining work activities, including laboratory inventory, restocking, ordering, and instrument calibration.
Participate in daily Agile stand-ups and weekly sprints with other members of the research team.
Bachelors (BS) with 6+ years, or Masters (MS) with 4+ years in cell biology, synthetic biology, genetics, biochemistry or related field.
3+ years of wet lab experience including operating scientific instrumentation such as thermocyclers, fluorescent microscopes, and gel electrophoresis systems.
Strong desire to learn multiple techniques and willingness to provide support as required.
Familiar with strict adherence to established SOP's.
Significant attention to detail and accuracy in following protocols.
Demonstrated ability to be highly productive in a fluid, fast-paced and teamwork environment.
Applying scientific principles and concepts to potential innovative platforms and disruptive technologies.
Strong cell culture skills with fundamental understanding of virus biology.
Experience with experimental design, data analysis and organization.
Hands-on lab knowledge and experience with current and next-gen genome engineering and sequencing technologies
Experience in designing DNA and RNA sequences (plasmids and maps, primers, guide RNA, siRNA).
Experience with organism design, systems biology, or cell modeling & simulation.
Ability to use single cell phenotyping (e.g. flow cytometry / FACS-based analysis)
Knowledge of and experience with molecular biology and/or synthetic biology experimental approaches
Experience with pre-clinical R&D for a cell or gene therapy product or technology development
Experience in preparing, running, and analyzing plate-based continuous and end-point functional assays.
Experience in interfacing with laboratory automation (e.g. automated liquid handlers) and data analysis software.
Experience with coding and running bioinformatics scripts (Python, numpy, scipy, pandas, jupyter).